I'm trying to collect feedback from my colleagues and the deadline is on 10-April-2012, 2.00pm. So in my mail I write:
Please email your feedback to me before 2.00pm, 10-April-2012(Tuesday).
Does it seems too commanding instead of being polite?
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If there is a tight deadline, as you seem to have, I always follow the sentence containing the deadline information with another sentence that says something like, "If you foresee a problem with replying by tomorrow, please let me know that as soon as possible."
This gives them a chance to tell you that the deadline is not feasible for them.